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Genes Associated with Detoxification

These genes affect how you process toxins, medications, and hormones. Variants don't break detox—they change how it works.

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Detox is a three-phase process.

Phase I activates compounds (CYP enzymes). Phase II conjugates them for excretion (GST, NAT, UGT, SULT).Phase III transports them out. The system only works when all phases are balanced. Fast Phase I + slow Phase II = activated toxins with nowhere to go.

The genes.

Organized by phase and function.

Phase I - Activation (CYP450 Enzymes)

Phase I enzymes activate compounds, making them more water-soluble but sometimes more reactive. The CYP family is the star here.

CYP1A2

Caffeine, drugs, toxins

Metabolizes caffeine, some medications, and environmental toxins. Fast metabolizers clear caffeine quickly; slow metabolizers feel it for hours.

Fast vs. slow affects caffeine sensitivity dramatically

CYP2D6

Drug metabolism

Metabolizes ~25% of all medications. Ultra-rapid, normal, intermediate, and poor metabolizer phenotypes exist.

Poor metabolizers may need different drug doses

CYP2C19

Drug metabolism

Metabolizes proton pump inhibitors, antidepressants, and clopidogrel. Variants affect drug efficacy.

Affects response to common medications

CYP1B1

Estrogen & toxin metabolism

Metabolizes estrogens and environmental toxins. Some metabolites are more harmful than others.

Affects estrogen metabolite profile

CYP3A4

Major drug metabolism

Metabolizes about 50% of all medications. Affected by grapefruit juice, St. John's Wort, and many drugs.

Mostly affected by inhibitors/inducers rather than genetics

Phase II - Conjugation

Phase II enzymes attach molecules to toxins, making them water-soluble for excretion. This is often the rate-limiting step.

GSTM1

Glutathione conjugation

Attaches glutathione to toxins. About 50% of people have a complete deletion (null genotype).

Null = no enzyme activity; very common

GSTP1

Glutathione conjugation

Another glutathione transferase. Important for clearing carcinogens and oxidative stress products.

Ile105Val affects enzyme activity

GSTT1

Glutathione conjugation

Metabolizes small reactive compounds. Null genotype common in ~20% of Caucasians, ~50% of Asians.

Null = no enzyme activity

NAT1/NAT2

Acetylation

Acetylate aromatic amines and drugs. Slow acetylators clear certain drugs and toxins slowly.

Fast vs. slow acetylator phenotypes

SULT1A1

Sulfation

Adds sulfate groups to hormones, drugs, and toxins. Important for estrogen and thyroid hormone metabolism.

Variants affect sulfation capacity

UGT1A1

Glucuronidation

Adds glucuronic acid for excretion. Gilbert's syndrome is a common UGT1A1 variant causing mild jaundice.

Gilbert's syndrome variant is common (~5-10%)

Phase III - Transport

Transporters move conjugated toxins out of cells and into bile or urine for elimination.

ABCB1 (MDR1)

Drug efflux

Pumps drugs and toxins out of cells. Affects drug absorption and brain penetration.

Affects drug levels in blood and tissues

ABCC2 (MRP2)

Bile transport

Exports conjugated toxins into bile. Variants can affect toxin elimination.

Affects biliary excretion

Antioxidant Defense

Detoxification generates oxidative stress. These genes manage the fallout.

SOD2

Mitochondrial antioxidant

Converts superoxide to hydrogen peroxide in mitochondria. The Ala16Val variant affects enzyme localization.

Val/Val may have lower mitochondrial activity

CAT

Hydrogen peroxide breakdown

Converts hydrogen peroxide to water. Works downstream of SOD.

Variants affect peroxide clearance

GPX1

Glutathione peroxidase

Uses glutathione to neutralize peroxides. Selenium-dependent enzyme.

Pro198Leu affects enzyme activity

NQO1

Quinone detoxification

Detoxifies quinones (from benzene, cigarette smoke). *2 variant has no enzyme activity.

*2/*2 = no enzyme function

Sulfur Metabolism

Sulfur pathways supply glutathione and sulfate for Phase II reactions.

CBSTranssulfuration

Converts homocysteine to cysteine (glutathione precursor). The gateway to sulfur metabolism.

Variants may affect sulfur compound production

SUOX

Sulfite oxidation

Converts toxic sulfite to sulfate. Molybdenum is the cofactor. Sulfite sensitivity may indicate issues here.

Rare mutations cause sulfite sensitivity

The GST null problem.

About 50% of people are missing GSTM1 entirely. Here's why that matters—and why it's not the disaster it sounds like.

The concern

GSTM1 null means you have no GSTM1 enzyme. You can't conjugate certain toxins via this pathway. Studies show increased cancer risk with high toxin exposure + null genotype.

The context

  • • Other GST enzymes (GSTP1, GSTT1) can partially compensate
  • • The null genotype is ancient and common—it's not a new mutation
  • • Risk only manifests with significant toxin exposure
  • • Reducing exposure matters more than trying to "fix" a missing gene

The strategy

If you have GST nulls: prioritize reducing toxin exposure, support glutathione synthesis, eat cruciferous vegetables (they upregulate other detox pathways), and ensure adequate selenium for GPX function.

What actually matters.

Genes set the baseline. These factors determine actual detox capacity.

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Glutathione Status

Phase II conjugation depends on glutathione. If it's depleted, even good genes can't compensate. Support synthesis with NAC, glycine, and glutamine.

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Toxin Exposure

The best detox gene variants can be overwhelmed by high exposure. Reduce inputs: clean food, filtered water, low-toxin products.

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Nutrient Cofactors

B vitamins, magnesium, zinc, selenium, and molybdenum are required cofactors. Deficiencies impair detox regardless of genetics.

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Bile Flow

Phase III requires bile to export toxins. Poor bile flow = toxin recirculation. Support with bitter foods, adequate fat, and liver support.

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Gut Health

The gut contains beta-glucuronidase that can reactivate excreted toxins. Dysbiosis impairs elimination.

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Phase Balance

Fast Phase I + slow Phase II = trouble. Activated intermediates accumulate. Balance matters more than speed.

"Detox genes don't determine your toxic load. They reveal how carefully you need to manage exposure."
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